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After Lorenzo Pellegrini pulled one back for Roma, Teun Koopmeiners immediately restored the two-goal advantage following a goalkeeping blunder by Rui Patricio.
Seventh-placed Atalanta moved within two points of sixth-placed Inter Milan and the Italian league’s Conference League spot.
Roma remained fifth, level on points with fourth-placed AC Milan, and four points ahead of Atalanta.
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After five straight seasons of playing in Europe, Atalanta didn’t qualify for any continental competitions for this season.
Roma faces Bayer Leverkusen in the Europa League semifinals.